Author/Authors :
Kuskur, Chethan Maheshwarappa Department of P.G. Studies and Research in Industrial Chemistry - Kuvempu University, India , Kumara Swamy, Bahaddurghatta Eshwaraswamy Department of P.G. Studies and Research in Industrial Chemistry - Kuvempu University, India , Jayadevappa, Honnali Departments of Chemistry - Sahyadri Science College, India , Shivakumar, Kuskur Departments of Chemistry - P.E.S Institute of Technology and Management, India
Abstract :
A sensitive and selective electrochemical method for the determination of Epinephrine using Eosin Y polymer film modified on the surface of carbon paste electrode was developed by using cyclic voltammetric technique. The Eosin Y polymer film modified electrode shows excellent electrocatalytic activity toward the oxidation of Epinephrine in 0.2 M phosphate buffer solution (PBS) of pH 7.0 at the sweep rate of 100 mV/s. The electrochemical parameter like the effect sweep rate and pH were investigated. The limit of detection was found to be 0.03 μM. Simultaneous investigations resulted in excellent potential difference between Epinephrine and Uric acid by using cyclic voltammetric technique and the interference study was carried out using the differential pulse voltammetric technique. This work provides a simple and easy approach to selectively determine EP in the presence of UA. The analytical performance of this biosensor has been evaluated for detection of epinephrine injection in serum as real sample
Keywords :
Epinephrine , Uric acid , Eosin , Cyclic Voltammetry , Electropolymerisation , Carbon Paste Electrode
