Author/Authors :
Hoseini, Mohammad Department of Pathobiology - Babol Branch, Islamic Azad University, Babol, Iran , Zaheri, Bahram Ali Department of Environmental Protection Semnan, Semnan, Iran , Adibi, Mohamad Ali Department of Environmental Protection Semnan, Semnan, Iran , Ronaghi, Hooman Department of Microbiology - Faculty of Specialized Veterinary Science - Science and Research Branch, Islamic Azad University, Tehran, Iran , Moshrefi, Amir Hossein Young Researchers and Elite Club - Babol Branch, Islamic Azad University, Babol, Iran
Abstract :
Background: The larval stages of Gasterophilus are obligate parasites in the gastrointestinal tract of equine account-able for pathologic ulcers in the Persian onager gastrointestinal. The aim of the current report was to study the histo-pathological change with G. pecorum larvae in the esophagus of a Persian onager.
Methods: This study was performed in Iranian Zebra propagation and breeding site in Khartouran National Park, southeast of Shahrud City, Semnan Province, Iran in 2014. Following a necropsy with specific refer to esophagus of one adult female Persian onager were transmitted to the laboratory. After autopsy, parasites collected from the esophagus were transmitted into 70% alcohol. For histopathological investigation, tissue samples were collected from the esophagus. The tissues were fixed in 10% buffered formalin, and conformity routine processing, there were stained with Hematoxylin and eosin.
Results: After clarity by lactophenol parasites were identified as G. pecorum. Microscopic recognition contained hyperemia, inflammatory cell infiltration, epithelial destruction, esophageal gland hyperplasia.
Conclusion: This is the first survey of G. pecorum and histopathological study in the Persian onager esophagus in the world.