Author/Authors :
Roth, Isabelle Institute of Pharmacology - Toxicology and Pharmacy - University of Veterinary Medicine Hannover , Schumacher, Stephan Institute of Pharmacology - Toxicology and Pharmacy - University of Veterinary Medicine Hannover , Basler,Tina Institute of Microbiology - University of Veterinary Medicine Hannover , Baumert, Kathrin Division of Immunodermatology and Allergy Research - Clinic for Dermatology - Allergy and Venereology - Hannover Medical School , Seitz, Jan-Marten Institute of Materials Science - Leibniz Universitat Hannover , Evertz, Florian Institute of Multiphase Processes - Leibniz Universitat Hannover , Muller, Peter Paul Helmholtz Centre for Infection Research , Baumer, Wolfgang MBS Department - NCSU College of Veterinary Medicine - Research Building , Kietzmann, Manfred Institute of Pharmacology - Toxicology and Pharmacy - University of Veterinary Medicine Hannover
Abstract :
Magnesium is currently under investigation as a
prospective biodegradable implant material. Biodegradation
of magnesium causes a release of magnesium,
hydroxide ions and hydrogen gas but it can also lead to the
formation of particulate debris. Implant-derived particles
may have immunotoxic effects. To investigate the influence
of magnesium-derived particles on the immune functions of
primary macrophages, up to 500 lg/ml magnesium or
magnesium corrosion particles were added to the cell culture
medium. No major effects were observed on cell viability
and on the release of the proinflammatory cytokine
tumor necrosis factor (TNF)a. In addition, the ability of
macrophages to stimulate proliferation of allogenic lymphocytes
in a mixed leukocyte reaction remained unaffected.
When macrophages were incubated with magnesium
particles and then infected with the apathogenic Mycobacterium
smegmatis, infection-induced TNFa secretion from
murine macrophages was inhibited but not from human
macrophages. However, the bactericidal activity of either
cell type was not influenced. In conclusion, magnesiumrelated
particles did not restrict the immune function of
macrophages, suggesting that magnesium implants and
corrosion particles derived thereof are highly biocompatible
and have a low inflammatory potential.
Keywords :
Infection , Immunotoxic , Degradation , Phagocytes , In vitro , Inflammation
