Title of article
Identification of Entamoeba histolytica by Molecular Method in Surface Water of Rasht City, Iran
Author/Authors
HEMMATI, Arash Dep. of Microbiology - Rasht Branch, Islamic Azad University, Rasht, Iran , HOOSHMAND, Elham Dep. of Veterinary - Rasht Branch, Islamic Azad University, Rasht, Iran , HOSSEINI, Mohammad Javad Molecular Biology Research Center - School of Medicine - Baqiyatallah University, Tehran, Iran
Pages
6
From page
238
To page
243
Abstract
Background: This study was conducted with the aim of determining surface water contamination with cysts of Enta-moeba histolytica using PCR in Rasht City, Northern Iran.
Methods: In this cross-sectional study, 49 water samples including 18 rivers and 6 wetlands were collected from dif-ferent regions near the city of Rasht in autumn of 2012. After filtration using 0.22 μm nitrate cellulose membrane fil-ters, the samples were examined using microscope and PCR method.
Results: In microscopic examination, four samples of the 49 samples were positive for cysts of E. (histolytica / dispar / muschkovskii). By using PCR method and molecular analysis, one sample was positive for E. histolytica.
Conclusion: In the molecular analysis, contamination by E. histolytica was proved in the waters of Rasht City. Further investigations including more samples and necessary preparations must be applied to prevent contamination.
Keywords
Entamoeba histolytica , PCR , Surface water
Journal title
Astroparticle Physics
Serial Year
2015
Record number
2420202
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