Author/Authors :
Fouladi, Salomeh Department of Immunology - Isfahan University of Medical Sciences , Adib, Minoo Department of Immunology - Isfahan University of Medical Sciences , Salehi, Mansoor Department of Genetics and Molecular Biology - Isfahan University of Medical Sciences , Karimzadeh, Hadi Department of Rheumatology - Medical School - Isfahan University of Medical Sciences , Bakhshiani, Zahra Department of Immunology - Isfahan University of Medical Sciences , Ostadi, Vajiheh Department of Immunology - Isfahan University of Medical Sciences
Abstract :
Background: HLA-B*27 is strongly associated with ankylosing spondylitis (AS). It
represents a family of alleles that differ among ethnic groups. Objective: The aim of
this study was to determine the distribution of HLA-B*27 alleles in AS patients and
healthy controls in Isfahan (Iran). Methods: Sixty AS patients and 430 healthy blood
donors were selected. All subjects were HLA-B*27 positive by flow cytometry. HLAB*
27 subtypes were determined by PCR-SSP. Results: Forty patients (66.7%) and 17
controls (3.95%) were HLA-B*27 positive. Subtypes detected by PCR-SSP were
B*2705, B*2702, B*2704 and B*2707. One patient was B*2702/B*2710. No significant difference was found in the distribution of these alleles between AS patients and controls. Conclusion: Although Caucasian subtypes are predominant among Iranians, this population is characterized by a combination of both specific Caucasian and Oriental subtypes. However such results should be interpreted carefully because of the small sample size in our investigation and definitive conclusion awaits more ethnicgroup studies.
