Author/Authors :
MASOUMI, Omid Dept. of Medical Parasitology and Mycology - School of Public Health, Tehran University of Medical Sciences - Tehran, Iran , SHAHZADI, Mahmoud Dept. of Cardiovascular Surgery - Imam Khomeini Hospita - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , KORDBACHEH, Parivash Dept. of Medical Parasitology and Mycology - School of Public Health, Tehran University of Medical Sciences - Tehran, Iran , ZAINI, Farideh Dept. of Medical Parasitology and Mycology - School of Public Health, Tehran University of Medical Sciences - Tehran, Iran , MAHMOUDI, Shahram Dept. of Medical Parasitology and Mycology - School of Public Health, Tehran University of Medical Sciences - Tehran, Iran , MAHMOUDI, Mahmoud 3. Dept. of Epidemiology and Biostatistics - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , BAHREINI, Hesamoddin Mehr Diagnostic Medical Laboratory, Karaj, Iran , SAFARA, Mahin Dept. of Medical Parasitology and Mycology - School of Public Health, Tehran University of Medical Sciences - Tehran, Iran , MIRHENDI, Hossein Dept. of Medical Parasitology and Mycology - School of Public Health, Tehran University of Medical Sciences - Tehran, Iran
Abstract :
Background: The aim of this study was to detect fungi in atherosclerotic plaques and investigate their possible role in atherosclerosis.
Methods: Coronary atherosclerotic plaques specimen were obtained from patients with atherosclerosis. Direct exami-nation, culture, histopathology study, PCR and sequencing were performed to detect/identify the mycotic elements in the plaques. Age, sex, smoking, obesity, hypertension, hyperlipidemia, family history of heart diseases and diabetes were considered and data were analyzed using Chi Square test by SPSS version 15.
Results: A total of 41 specimens were analyzed. Direct examination for fungal elements was negative in all cases but in culture only one specimen grew as a mold colony. The presence of fungal elements were confirmed in 6 and 2 tissue sections stained by Gomori methenamine silver and Hematoxylin and Eosin methods, respectively. Using PCR, 11 cases were positive for fungi. The DNA sequence analysis of six positive specimens which were randomly selected revealed fungi as Candida albicans (n=3), Candida guilliermondii (n=2) and Monilia sp. (n=1).
Conclusion: A significant association between the presence of fungi in atherosclerotic plaques and severity of athero-genesis and atherosclerotic disease was not found. This could be due to limited numbers of patients included in our study. However, the presence of fungal elements in 26.8% of our specimens is considerable and the results does not exclude the correlation between the presence of fungi with atherosclerosis and coronary artery disease.
Keywords :
Atherosclerosis , Coronary disease , Candida , Fungi
