Title of article :
Incidence of Human T-Lymphotropic Virus Type 1 (HTLV-1) among Blood Donors from Ilam, Iran
Author/Authors :
Falahi, Shahab Dept. of Virology Faculty of Medical science - Tarbiat Modares University , Sabahi, Farzaneh Dept. of Virology Faculty of Medical science - Tarbiat Modares University , Zainali, Majid Blood Transfusion Organization of llam , Kiani, Jala Dept. of Virology - Faculty of Medical science - Iran University of medical science , Ranjbari, Javad Dept of Biotechnology - Tabriz University , Khalafi, Sakineh Blood Transfusion Organization of llam
Abstract :
llam city is the center of llam province, a western province of Iran. In the present study, HTLV screening were performed among 960 serum samples from blood donors by using enzymelinked immunosorbent assay (ELISA), from March/21/2006 to May/27/2007. In primary screening, 3 (0.003125) samples were positive (two males and one female). Positivity of the samples were confirmed by Western blot (
WB) analysis. The WB results indicated that, of 3 positive ELISA specimens, 2 specimens (66.6%) were HTLV-1, and 1 specimen (33.3%) could not be confirmed. For further evaluation, the HTLV-1-WB positive samples and HTLV-1-seropositive but WB-negative sample were examined by PCR. Results showed that the HTLV-1 WB-positive samples were determined as HTLV-1 and the negative sample could not be confirmed for HTLV-1 by PCR. The incidence of HTLV-1 infection in our study was 2/960 (0.00208) among blood bank donors, which confirms the city of llam as a non-endemic area, compared to other regions in Iran (Mashhad: 0.77% and Mazandaran: 1.6%) and in the world.
Keywords :
HTLV , incidence , Blood donor , Western Blot , PCR , Ilam
Journal title :
Astroparticle Physics
