Author/Authors :
Valadkhani, Z Department of Parasitology - Pasteur Institute of Iran, Tehran, Iran , Kazemi, F Department of Parasitology - Pasteur Institute of Iran, Tehran, Iran , Assmar, M Department of Parasitology - Pasteur Institute of Iran, Tehran, Iran , Amirkhani, A Department of Parasitology - Pasteur Institute of Iran, Tehran, Iran , Esfandeari, B Amole Research Center - Pasteur Institute of Iran, Amole, Iran , Lotfi, M Amole Research Center - Pasteur Institute of Iran, Amole, Iran , Ghobadirad, S Amole Research Center - Pasteur Institute of Iran, Amole, Iran , Hassan, N Department of Parasitology - Pasteur Institute of Iran, Tehran, Iran , Aghighi, Z Department of Parasitology - Pasteur Institute of Iran, Tehran, Iran
Abstract :
Background: Trichomoniasis is an extremely common sexually transmitted infection (STI)
worldwide and is associated with important public health problems, including amplification of
HIV transmission. This disease is in forms of symptomatic and asymptomatic in women and may
depend on host as well as parasite variables. Most of the studies reported from females are based
on examination of vaginal secretions and urine samples by direct smear and culture in modified
Diamond's media. The aim of this study was checking the samples, which were negative by direct
smear and culture, with PCR technique.
Methods: The urine samples and vaginal discharge of patients attending Gynecology Clinics of
Mazandaran Province, Iran with different symptoms rechecked for Trichomonas vaginalis by
PCR technique using primers targeting a conserved region of the beta-tubulin genes of the parasite.
Data were analyzed by Epi Info software program
Results: Out of 161 negative samples by direct smear and culture, seven samples (4.3%) were
positive by PCR technique.
Conclusion: Diagnosis of trichomoniasis by PCR is a sensitive and specific method that could
play important role to help the physicians for properly treatment and control of infection.
