Author/Authors :
NIYYATI, Maryam Dept. of Medical Parasitology and Mycology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , SASANI, Roghayeh Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , MOHEBALI, Mehdi Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , GHAZIKHANSARI, Mahmoud Dept. of Pharmacology - School of Medicine - Tehran University of Medical Sciences, Tehran, Iran , KARGAR, Faranak Rajaie Cardiovascular Medical and Research Center - Iran University of Medical Sciences, Tehran, Iran , HAJIALILO, Elham Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran , REZAEIAN, Mostafa Dept. of Medical Parasitology and Mycology - School of Public Health - Tehran University of Medical Sciences, Tehran, Iran
Abstract :
Background: This study aimed to investigate the anti-Acanthamoeba effects of the
most used marketed disinfecting solutions in Iran. Moreover, the efficacy of some
nano-compounds was tested against pathogenic Acanthamoeba.
Methods: The present study was conducted in the School of Public Health, Tehran
University of Medical sciences, Tehran, Iran during 2015-2016. Cysts of Acanthamoeba
T4 genotype (7 x 104 /ml) mixed at the equal volume with contact lens
solutions including Opti-free, Ginza, ReNu, Maxima, Light, and Cyclean for the
recommended time by the manufacturers. Nano-silver and nano-gold compounds
were also treated with the amoebae. Chlorhexidine 0.02% and normal saline were
used as positive and negative controls, respectively. Dead and alive amoebae were
determined using vital stain and suspension was cultured in non-nutrient agar. The
entire process was repeated at least three times.
Results: In none of the solutions in the manufacturer's brochure recommended
time, full cytotoxic effect was observed on the cysts of Acanthamoeba. Opti free express
solution destroyed the cysts after 6 days. Nanosilver and nano-gold compounds
showed no cytotoxic effect on the cysts of Acanthamoeba.
Conclusion: None of the Nanoparticles compounds as well as contact lenses disinfecting
solutions which studied was effective on Acanthamoeba cysts in the manufacturer's
brochure recommended time. However, continuing study on Nano-silver
and Nano-gold compounds to find effective ingredients against Acanthamoeba are
highly recommended.
