Phytochemical and Antimicrobial Analysis on the Extracte of Oroxylum indicum Linn. Stem-Bark

The stem bark of Oroxylum indicum (Fam. Bignoniaceae) was extracted with methanol. The methanol extract was fractionated with pet ether, chloroform, hexane and carbon tetrachloride using modified Kupchan partitioning method. Extensive chromatographic separation and purification with the organic solvent was done. One compound was extracted from the n-hexane fraction of stem bark of O. indicum which was then confirmed as flavonoid type of compound by using 1H NMR spectrometric analysis. Three fractions (hexane, carbon tetrachloride, chloroform), obtained from methanolic crude extract, were tested for antimicrobial activity by standard disc diffusion method. The extracts were found to show antimicrobial activities towards the gram-positive and gram-negative bacteria, and some fungi. The results were compared with same microbial strains against a standard antibiotic disc of ampicillin (10 μg/disc).