Title of article :
Detection of Toxoplasma gondii Antigens in Sera and Urine of Experimentally Infected Mice by Capture ELISA
Author/Authors :
Foroghi parvar, F Dept. of Medical Parasitology and Mycology - School of Public Health - Medical Sciences University of Tehran , Keshavarz, H Dept. of Medical Parasitology and Mycology - School of Public Health - Medical Sciences University of Tehran , Shojaee, S Dept. of Medical Parasitology and Mycology - School of Public Health - Medical Sciences University of Tehran
Abstract :
Background: Toxoplasma gondii is an apicomplexan parasite which infects a broad range of hosts. The classical diagnosis of toxoplasmosis relies on serological methods. Detection of parasite or its components could be useful tool for early diagnosis of the infection.Methods: Fifty mice were infected by the intraperitoneal route with 5000 tachyzoites of T. gondii RH strain. Five of them sacrificed every day from day 1 up to day 7 post infection. Sera and urine of mice were tested by capture ELISA.Results: T.gondii antigens were detected from 3rd and 2nd day in serum and urine, respectively, after infection until their death on day 7.Conclusion: Antigenemia detection of antigens of parasite was possible in a short period of acute infection with T. gondii by capture ELISA.
Keywords :
Toxoplasma gondii , Antigenemia , Capture ELISA
Journal title :
Astroparticle Physics
