Author/Authors :
Ghorbian, S Department of Biology - Science and Research Branch, Islamic Azad University , Saliminejad, K Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR , Sadeghi, MR Monoclonal Antibody Research Center - Avicenna Research Institute, ACECR , Javadi, GhR Department of Biology - Science and Research Branch, Islamic Azad University , Kamali, K Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR , Amirjannati, N Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR , Bahreini, F Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR , Edalatkhah, H Reproductive Biotechnology Research Center - Avicenna Research Institute, ACECR , Khorram Khorshid, HR Genetic Research Center - University of Social Welfare and Rehabilitation Science, Tehran, Iran
Abstract :
Background: To date, the role of male factor contributing in evaluation of spontaneous recurrent pregnancy loss
(RPL) has been less investigated and there is discrepancy in the role of Y chromosome microdeltions in RPL.
Therefore, the current study was designed to examine whether Y chromosome microdeletions were associated
with RPL in an Iranian population.
Methods: One hundred men from couples, experiencing three or more RPLs, and one hundred normal men from
couples with at least one child and no history of miscarriages as control group were included. Genomic DNA was
extracted from peripheral blood and tested for Y chromosome microdeletions in AZFa, AZFb and AZFc regions
using two multiplex PCR.
Results: None of the men in the case and control groups had any microdeletions in the AZFa, AZFb and AZFc
regions.
Conclusion: It seems that Y chromosome microdeletion is not associated with recurrent pregnancy loss,
therefore performing this test in Iranian couples with RPL is not recommended.
Keywords :
Recurrent pregnancy loss , Miscarriages , Y chromosome microdeletion , AZF regions , Infertility
