Title of article
Comparison of Cytotoxic Activity of Bile on HepG2 and CCRF-CEM Cell Lines: An in Vitro Study
Author/Authors
Azarpira, Negar Organ Transplant Research Center - Nemazee Hospital - Shiraz University of Medical Sciences - Shiraz , Rastegar, Fatemeh Organ Transplant Research Center - Nemazee Hospital - Shiraz University of Medical Sciences - Shiraz , Amiri, Maryam Organ Transplant Research Center - Nemazee Hospital - Shiraz University of Medical Sciences - Shiraz , Esfandiari, Elaheh Organ Transplant Research Center - Nemazee Hospital - Shiraz University of Medical Sciences - Shiraz , Geramizadeh, Bita Organ Transplant Research Center - Nemazee Hospital - Shiraz University of Medical Sciences - Shiraz
Pages
5
From page
266
To page
270
Abstract
The aim of this study was to examine the effect of crude bile on the human HepG2 and CCRF-CEM cell lines. Cells were exposed to different dilutions of bile. Antiproliferative effects were determined by the cytotoxic MTT assay. Cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Bile administration resulted in dose-dependent cytotoxicity in both HepG2 and CCRF-CEM cell lines. Incubated cells exhibited morphologic features of apoptosis. Bile has significant cytotoxic activity in HepG2 and CCRF-CEM cancer cells via induction of apoptosis. The mechanism of apoptosis needs to be further evaluated. It may have clinical utility in the treatment of cancer after in vivo confirmation of activity.
Keywords
Bile , Neoplasms , Cytotoxicity
Journal title
Astroparticle Physics
Serial Year
2012
Record number
2422148
Link To Document