Author/Authors :
Sreenivasa, Gopalappa Molecular Reproductive and Human Genetics Labratory - Department of Studies in Zoology - University of Mysore, Manasagangothri, Mysore, India , Kavitha, Papanna Molecular Reproductive and Human Genetics Labratory - Department of Studies in Zoology - University of Mysore, Manasagangothri, Mysore, India , Satidevi Vineeth, Venugopal Molecular Reproductive and Human Genetics Labratory - Department of Studies in Zoology - University of Mysore, Manasagangothri, Mysore, India , Kumar Channappa, Sharath Mediwave IVF and Fertility Research Center and Semen Bank, Mysore, Karnataka, India , Naik Malini, Suttur Srikanta Molecular Reproductive and Human Genetics Labratory - Department of Studies in Zoology - University of Mysore, Manasagangothri, Mysore, India
Abstract :
Background: Infertility is a condition associated with multiple etiologies. Sperm nuclear chromatin decondensation is one of the
important events that occur during fertilization. Abnormal spermatogenesis leads to improper protamine package and chromatin
condensation. The aim of the study was to analyze and understand the levels of fertilization capacity and nuclear stability of the
spermatozoa in different infertile subgroups. Material and Methods: A total of 65 infertile males and 24 fertile males were employed
in the study. Infertile subjects were classified into different groups according to the World Health Organization (WHO) protocol.
In this study, in vitro nuclear chromatin decondensation status was assessed in different subgroups of infertile males. The obtained
data was then statistically analyzed. Results: Decreased sperm chromatin decondensation was observed in different infertile
subgroups compared to the control group (p < 0.05). Spermatozoa with swollen head indicated a positive response and unswollen
head indicated a negative response. Conclusion: This study asserts that abnormal nuclear decondensation is a potential factor that
diminishes the fertilizing capacity of the sperms among different subgroups of infertile males.
Keywords :
Nuclear Chromatin Decondensation, , Protamines , DNA Damage , Infertility
