Author/Authors :
Pakzad, Iraj Department Microbiology - Faculty of Medicine and Clinical Microbiology Research Center - Ilam University of Medical Sciences , Ghafourian, Sohbhan Department Microbiology - Faculty of Medicine and Clinical Microbiology Research Center - Ilam University of Medical Sciences , Taherikalani, Morovat Department Microbiology - Faculty of Medicine and Clinical Microbiology Research Center - Ilam University of Medical Sciences , sadeghifard, Norkhoda Department Microbiology - Faculty of Medicine and Clinical Microbiology Research Center - Ilam University of Medical Sciences , Abtahi, Hamid Department Microbiology - Faculty of Medicine and Molecular Medicine Research Center - Arak University of Medical Sciences , Rahbar, Mohammad Department Microbiology - Iranian Reference Health Laboratory, Tehran , Mansory Jamshidi, Neda Department Microbiology - Karaj Islamic Azad University
Abstract :
Objective(s) Extensive use of quinolones has been associated with raising level of resistance. In the current, we focused on assessing the prevalence of Escherichia coli resistance to quinolones and frequency of qnrA, qnrB and qnrS in non ESBLs (extended spectrum beta-lactamases) and ESBLs producing E. coli with blaSHV and
blaTEM. Materials and Methods One hundred and fifty E. coli isolates were identified during Mar. 2007 to Apr. 2008 in Milad (Tehran) hospital. They were tested for ESBLs production as well as quinolone resistance. PCR was performed for detection of blaSHV and blaTEM as well as qnrA, B and S. Results Of 150 isolates, forty-two (28%) ESBLs producing and one hundred and eight (72%) non-ESBLs producing
E. coli were identified. 64.2% (n= 24) of E. coli producing ESBLs and 4.62% (n= 5) of non-ESBLs E. coli were resistance to ciprofloxacin. 95.2% (n= 40) and 26.1% (n= 11) of the isolates harbored blaTEM and blaSHV, respectively. 23.8% (n= 10) had both genes. 37.5% (n= 9) and 20.8% (n= 4) of ESBLs producing E. coli were positive for qnrA and qnrB respectively. qnrS was not identified in any isolate. Conclusion
Our study showed high frequency of ESBLs producing E. coli as well as quinolone resistance genes (qnrA, qnrB) in Milad hospital.
