Author/Authors :
Baghbani, Fatemeh Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Raoofian, Reza Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Hasanzadeh Nazarabadi, Mohammad Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Hamzehloei, Tayebeh Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Soukhtanloo, Mohammad Department of Clinical Biochemistry - School of Medicine - Mashhad University of Medical Sciences , Heidari, Mansur Department of Medical Genetics - School of Medicine - Tehran University of Medical Sciences , Afsharzadeh, Morteza Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Shekouhi, Sahar Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Moradi, Fahimeh Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Sarli, Abdol-Azim Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences , Zavar-Reza, Javad Department of Clinical Biochemistry - School of Medicine - Shahid Sadoughi University of Medical Sciences, Yazd , Mojarrad, Majid Department of Medical Genetics - School of Medicine - Mashhad University of Medical Sciences
Abstract :
Objective(s): Hypoxia is a serious challenge for treatment of solid tumors. This condition has been manifested to exert significant therapeutic effects on glioblastoma multiform or (WHO) astrocytoma grade IV. Hypoxia contributes numerous changes in cellular mechanisms such as angiogenesis, metastasis and apoptosis evasion. Furthermore, in molecular level, hypoxia can cause induction of DNA breaks in tumor cells. Identification of mechanisms responsible for these effects can lead to designing more efficient therapeutic strategies against tumor progression which results in improvement of patient prognosis. Materials and Methods: In order to identify more hypoxia regulated genes which may have a role in glioblastoma progression, cDNA-AFLP was optimized as a Differential display method which is able to identify and isolate transcripts with no prior sequence knowledge. Results: Using this method, the current study identified 120 Transcription Derived Fragments (TDFs) which were completely differentially regulated in response to hypoxia. By sequence homology searching, the current study could detect 22 completely differentially regulated known genes and two unknown sequence matching with two chromosome contig and four sequence matches with some Expressed Sequence Tags (ESTs). Conclusion: Further characterizing of these genes may help to achieve better understanding of hypoxia mediated phenotype change in tumor cells.
