Title of article :
Isolation of a novel lectin from the dorsal spines of the devil stinger, Inimicus japonicus
Author/Authors :
Nakagawa, Hideyuki Division of Environmental Symbiosis - Graduate School of Integrated Arts and Sciences - Tokushima University , Nagasaka, Kuniko , Sakai, Hitomi Division of Environmental Symbiosis - Graduate School of Integrated Arts and Sciences - Tokushima University , Edo, Kozue Junior College Division - Department of Science for Human Health - Shikoku University , Shinohara, Mitsuko Department of Pharmacology - Osaka Dental University , Ohura, Kiyoshi
Abstract :
A novel lectin was purified from the dorsal spines of the devil stinger, Inimicus japonicus using a
combination of affinity chromatography techniques. A single band was detected on a native PAGE gel with a
relative molecular mass of 97 kDa. The N-terminal partial amino acid of the intact 75 kDa subunit of the
97 kDa lectin was found to be DHEDS. The agglutination of rabbit erythrocytes by the 97 kDa lectin was
inhibited most effectively by methyl a-D-mannoside. The 97 kDa lectin stimulated mitogenesis in murine
splenocytes. This is the first study to examine the dorsal lectin of I. japonicus and one of the very few studies
on venom lectins from venomous scorpaeniform fish. These results suggest that the devil stinger, I. japonicus,
may be a novel resource for biologically active substances.
Keywords :
Murine splenocytes , Mitogenic activity , Agglutination , Lectin , Dorsal spine , Inimicus japonicus , Devil stinger
Journal title :
Astroparticle Physics
