Fractionation of Anticholesterol Bioactive Compounds from Bekasam (Indonesian Fermented Fish Product)

Bekasam functions as an inhibitor of HMG-CoA reductase. Fractionation was required to determine the bioactive peptide which functions as a HMG-CoA reductase inhibitor. Steps taken for this research were the production of bekasam used salt (15%), rice (15%) and Lactobacillus acidophilus as a culture starter, extraction and fractionation of bekasam to assay its HMG-CoA reductase inhibition. The results showed that six fractions from bekasam extract had different inhibition activity. The fraction of bekasam extract without evaporation (F1) contained 3 peptides (peptide of 7.69 kD; 10.71 kD and 20.22 kD). Extract free supernatant fraction (F2) contained 4 peptides (peptide of 7.69 kD; 10.71 kD; 20.22 kD and 35.38 kD). Fractions of bekasam extract in the F3 contained 2 peptides (7.69 kD and 10.71 kD). Furthermore, fractionation in the F4 can separate only one peptide band with molecular weight 7.69 kD. Peptides were not discovered in the F3 and F4 fraction while F6 and F4 fractions had the higher inhibition fraction to HMG-CoA reductase activity (92.86%). There was peptide 7.69 kD in F4 fraction and lovastatin (148.30 ppm) in F6 fraction.