Title of article :
Folate-Conjugated Gold Nanoparticles (Synthesis, Characterization and Design for Cancer Cells Nanotechnology-based Targeting)
Author/Authors :
Hashemian, A.R Department of Medical Physics - Mashhad University of Medical Sciences , Eshghi, H Department of Chemistry - Ferdowsi University of Mashhad , Mansoori, G.A Departments of Bioengineering - Chemical Engineering & Physics - University of Illinois at Chicago , Shakeri-Zadeh, A Department of Medical Physics - Iran University of Medical Science , Mehdizadeh, A.R Department of Medical Physics - Shiraz University of Medical Sciences
Abstract :
A new folate-conjugated gold nanoparticle (AuNP) has been designed to selectively target the folate receptor that is overexpressed on the surface of tumoral cells. For this purpose, we made 4-aminothiophenol, as a bifunctional linker to react with HAuCl4 in the presence of sodium borohydride and it was binded to the AuNP surface through its thiol group. Then, we conjugated amino-terminated nanoparticles to folic acid with an amide-linkage formation. we evaluated the specific interaction between the folic acid and AuNP by the corresponding observed characteristic bands in the UV-vis and FTIR spectra. Transmission electron microscopic (TEM) images reveal the spherical AuNPs formation induced by the bifunctional linker. Powder X-ray diffraction (XRD) patterns confirmed the metallic face-centered cubic (FCC) lattice structure with (111), (200), (220), and (311) crystal planes We estimated the average size of the conjugated nanoparticles to be about 5 nm by TEM and XRD. The Elemental analysis and atomic absorption showed 59 % organic molecules on the surface of AuNPs
Keywords :
4-Aminothiophenol , AuNp , Cancer Cell Targeting , ConJugated Nanoparticle , Folate , Folic Acid , Nanotechnology
Journal title :
Astroparticle Physics
