Title of article :
The effects of crocetin, extracted from saffron, in chemotherapy against the incidence of multiple drug resistance phenotype
Author/Authors :
Neyshaburinezhad, Navid Biopharmaceutics and Pharmacokinetic Division - Department of Pharmaceutics - Faculty of Pharmacy - Tehran University of Medical Sciences, Tehran , Hashemi, Maryam Nanotechnology Research Center - Pharmaceutical Technology Institute - Mashhad University of Medical Sciences , Ramezani, Mohammad Pharmaceutical Research Center - Pharmaceutical Technology Institute - Mashhad University of Medical Sciences, Mashhad - Department of Pharmaceutical Biotechnology - School of Pharmacy - Mashhad University of Medical Sciences , Arabzadeh, Sepideh Biotechnology Research Center - Pharmaceutical Technology Institute - Mashhad University of Medical Sciences , Behravan, Javad Department of Pharmaceutical Biotechnology - School of Pharmacy - Mashhad University of Medical Sciences - Biotechnology Research Center - Pharmaceutical Technology Institute - Mashhad University of Medical Sciences , Kalalinia, Fatemeh Biotechnology Research Center - Pharmaceutical Technology Institute - Mashhad University of Medical Sciences
Abstract :
Objective(s): Crocetin, one of the main substances of saffron extract, has anti-cancer effects. Drug resistance proteins (e.g. MRP1 and MRP2) are important reasons for the failure of cancer therapy. We intended to investigate the efficacy of crocetin on MRP1 and MRP2 activity in human ovarian cisplatinresistant Materials and Methods: The cytotoxic effect of crocetin was evaluated by the MTT assay. The efficacy of crocetin on MRP1 and MRP2 expression at mRNA level was studied by real-time RT-PCR. The effect of crocetin on the activity of MRP transporters was determined by drug efflux assay. Results: Crocetin decreased cell proliferation in the A2780 (IC50: 183±7 μM) and A2780-RCIS (IC50: 316±9 μM). Crocetin decreased the expression level of MRP1 (22±2 %) and MRP2 (48±8 %) in A2780- RCIS and inhibited MRP pumps function directly in A2780 (44±1 %) and A2780-RCIS (88±10 %) and indirectly in A2780 (32±2 %) and A2780-RCIS (48±15 %) respectively. Conclusion: Our findings showed that crocetin could quench drug resistance through modulation of MRP transporters in the drug resistant human ovarian cancer cells.
Keywords :
A2780 , A2780-RCIS , Crocetin , MRP , Ovarian cancer cell line
