Author/Authors :
Badbarin, Ali Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran , Jalilian, Amir Reza Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran , Yousefnia, Hassan Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran , Mazidi, Mohammad Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran , Bolourinovin, Fatemeh Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran
Abstract :
Introduction: The endemic occurrence of new strains of tuberculosis around the globe has initiated new research on
developing diagnostic radiopharmaceutical based on antibiotics.
Methods: Tc-99m labeled streptomycin was prepared using freshly milked 99mTcO4
- and various concentrations of
streptomycin, stannous chloride at various temperatures in high yield radiochemical purity shown by HPLC/RTLC.
Results: At various pHs tested (3-10) the best labeling yield at optimized conditions were at 6-8 range, however pH.7 was
considered the best. The radiochemical purity at the best optimized conditions was >95% as shown by RTLC and >97%
using HPLC (specific activity; 1-3 GBq/mmole). The tracer proved to be stable in the final product and in presence of human
serum at 37°C up to 2h. However, biodistribution and high resolution SPECT studies in wild type mice showed instability of
the complex due to high stomach and thyroid activity uptake.
Conclusion: Regardless the reported methods in the literature the direct 99mTc-labeling of aminoglycosides did not proved a
suitable tracer.
Keywords :
Technetium-99m , Streptomycin , SPECT , Biodistribution
