Author/Authors :
Táccio de Miranda Candeiro, George Department of Restorative Dentistry - Faculty of Dentistry, University of São Paulo - São Paulo, Brazil , Akisue, Eduardo Discipline of Endodontics - Faculty of Dentistry, University Santa Cecilia, Santos Brazil , Campelo Correia, Fabrícia Department of Dental Clinic - Faculty of Pharmaceutics, Dentistry and Nursy - Federal University of Ceará - Fortaleza, Brazil , dos Santos Sousa, Edmilson Department of Dental Clinic - Faculty of Pharmaceutics, Dentistry and Nursy - Federal University of Ceará - Fortaleza, Brazil , Sampaio do Vale, Mônica Department of Dental Clinic - Faculty of Pharmaceutics, Dentistry and Nursy - Federal University of Ceará - Fortaleza, Brazil , Faga Iglecias, Elaine Department of Restorative Dentistry - Faculty of Dentistry, University of São Paulo - São Paulo, Brazil , Gavini, Giulio Department of Restorative Dentistry - Faculty of Dentistry, University of São Paulo - São Paulo, Brazil
Abstract :
Introduction: The aim of the present research was to evaluate the effectiveness of 5% malic acid, 17% EDTA and 10% citric acid solutions used to disinfect gutta-percha cones contaminated by Enterococcus faecalis (ATCC 29212). Methods and Materials: Two hundred and ten previously sterilized gutta-percha cones were contaminated with E. faecalis at concentration of 1.5×108 CFU/mL. The cones were immersed in 5% malic acid, 17% EDTA, 10% citric acid, 1% NaOCl and 2.5% NaOCl for 1, 5 and 10 min. Then each cone was kept in Eppendorf tubes containing BHI sterile solution at 37°C for 48 h. The presence of turbidity in BHI solution was analyzed. The results were statistically analyzed by Kruskal-Wallis test and 5% Dunn comparisons. P-value was considered statistically significant when P<0.05. Results: Regardless of exposure time, 1% NaOCl and 2.5% NaOCl were the most effective agents for rapid disinfection of gutta-percha cones (P<0.001). All specimens immersed in experimental demineralized solutions presented bacterial growth (P>0.05). Conclusion: Demineralized solutions tested were not effective for elimination of Enterococcus faecalis on the surface of gutta-percha cones.
Keywords :
Chemical Substances , Disinfection , Gutta-Percha , Irrigating Solution
