Comparative Scanning Electron Microscopic Study of the Marginal Adaptation of Four Root-End Filling Materials in Presence and Absence of Blood

Objectives: The aim of this study was to evaluate marginal adaptation of mineral tri- oxide aggregate (MTA), calcium enriched mixture (CEM) cement, Biodentine and BioAggregate in presence of normal saline and human blood. Materials and Methods: In this in-vitro experimental study, 80 extracted single- rooted human teeth were instrumented and filled with gutta-percha. After resecting the root-end, apical cavity preparation was done and the teeth were randomly divided into four groups of 20 (a total of eight subgroups). Root-end filling materials were placed in three-millimeter root-end cavities prepared ultrasonically. Half the speci- mens in each group were exposed to normal saline and the other half to fresh whole human blood. After four days, epoxy resin replicas of the apical portion of samples were fabricated and scanning electron microscopy analysis was performed to find gaps in the adaptation of the root-end filling materials at their interface with dentin. The Kruskal-Wallis and Mann-Whitney tests were used for statistical analysis of data with P<0.05 level of significance. Results: There were no significant differences in marginal adaptation of the eight tested groups (P>0.05). Conclusion: Based on the results, blood contamination does not affect the marginal adaptation of MTA, CEM cement, Biodentine or BioAggregate.