Author/Authors :
Ghorbanzadeh, Abdollah Tehran University of Medical Sciences, Tehran , Aminsobhani, Mohsen Tehran University of Medical Sciences, Tehran , Khoshzaban, Ahad Dental Faculty, Tehran University of Medical Sciences, Tehran , Abbaszadeh, Armin , Bolhari, Behnam Tehran University of Medical Sciences, Tehran , Ghorbanzadeh, Atiyeh Semmelwies University - Budapest, Hungary , Shamshiri, Ahmad Reza Department of Community Oral Health - School of Dentistry, Tehran University of Medical Sciences, Tehran
Abstract :
Objectives: The aim of this study was to assess the cytotoxic effects and osteogenic activity of recombinant human bone morphogenetic protein (rhBMP2) and nano-hydroxyapatite (n- HA) adjacent to MG-63 cell line.
Materials and Methods: To assess cytotoxicity, the 4,5-dimethyl thiazolyl-2,5-diphenyl tetrazolium bromide (MTT) assay was used. Alkaline phosphatase (ALP) activity and oste- ogenic activity were evaluated using Alizarin red and the von Kossa staining and analyzed by one-way ANOVA followed by Tukey’s post hoc test.
Results: The n-HA/calcium sulfate (CS) mixture significantly promoted cell growth in comparison to pure CS. Moreover, addition of rhBMP2 to CS (P=0.02) and also mixing CS with n-HA led to further increase in extracellular calcium production and ALP activity (P=0.03).
Conclusion: This in vitro study indicates that a scaffold material in combination with an osteoinductive material is effective for bone matrix formation.
Keywords :
Calcium sulfate , Cytotoxicity , Nanohydroxyapatite , Recombinant human bone morphogenetic protein
