Author/Authors :
Sharma, Meenakshi Department of Pedodontics and Preventive Dentistry - Government Dental College - Jaipur, Rajasthan, India , Sharma, Sanjay Medical Officer - Jaipur, Rajasthan, India , G. Reddy, Y Department of Orthodontics and Dentofacial Orthopedics - NIMS Dental College, Jaipur - Rajasthan, India , Mittal, Rakesh Department of Pedodontics and Preventive Dentistry - NIMS Dental College, Jaipur - Rajasthan, India , Agarwal, Vishal Department of Conservative Dentistry - NIMS Dental College, Jaipur, India , Singh, Chanchal Department of Pedodontics and Preventive Dentistry - NIMS Dental College, Jaipur - Rajasthan, India , Singh, Amandeep Department of Public Health Dentistry - NIMS Dental College, Jaipur
Abstract :
Objectives: This study was undertaken to evaluate the viability of periodontal ligament (PDL) cells of avulsed teeth in three different storage media.
Materials and Methods: Forty-five premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups based on storage media used [Group I: milk (control); Group II: aloe vera (experimental); Group III: egg white (experimental)]. Following extractions, the teeth were placed in one of the three differ- ent storage media for 30 minutes, following which the scrapings of the PDL from these teeth were collected in Falcon tubes containing collagenase enzyme in 2.5 mL of phos- phate buffered saline. The tubes were subsequently incubated for 30 minutes and cen- trifuged for five minutes at 800 rpm. The obtained PDL cells were stained with Trypan Blue and were observed under optical microscope. The percentage of viable cells was calculated.
Results: Aloe vera showed the highest percentage of viable cells (114.3±8.0), followed by egg white (100.9±6.3) and milk (101.1±7.3).
Conclusion: Within the limitations of this study, it appears that aloe vera maintains PDL cell viability better than egg white or milk.
