Author/Authors :
Emami, Raheleh Department of Oral and Maxillofacial Radiology - Faculty of Dentistry, Semnan University of Medical Sciences, Semnan , Khedmat, Sedigheh Tehran University of Medical Sciences, Tehran , Pirmoazen, Salma Department of Endodontics - School of Dentistry, Tehran University of Medical Sciences - International Campus, Tehran , Honardar, Kiamars Department of Endodontics - School of Dentistry, Shahed University of Medical Sciences, Tehran
Abstract :
Objectives: The aim of this study was to compare apical sealing ability of alpha and beta phases of gutta-percha by means of bacterial leakage model.
Materials and Methods: Fifty single-rooted human premolars were selected. The root canals were prepared with Mtwo rotary instruments up to apical #35.04. Forty teeth were randomly divided into two groups (n= 20). The root canals were obturated by alpha phase (G1) and beta phase (G2) of gutta-percha and AH26 sealer, respectively, with warm vertical compaction technique. Ten teeth served as positive (n=8) and negative (n=2) control groups. Then, the specimens were sterilized with ethylene oxide gas. Bacterial suspension of Enterococcus faecalis (E. faecalis) in 0.5 McFarland concentration was prepared. All teeth
were mounted in plastic vial caps containing Muller Hinton broth and then exposed to Objectives: The aim of this study was to compare apical sealing ability of alpha and beta phases of gutta-percha by means of bacterial leakage model.
Materials and Methods: Fifty single-rooted human premolars were selected. The root canals were prepared with Mtwo rotary instruments up to apical #35.04. Forty teeth were randomly divided into two groups (n= 20). The root canals were obturated by alpha phase (G1) and beta phase (G2) of gutta-percha and AH26 sealer, respectively, with warm vertical compaction technique. Ten teeth served as positive (n=8) and negative (n=2) control groups. Then, the specimens were sterilized with ethylene oxide gas. Bacterial suspension of Enterococcus faecalis (E. faecalis) in 0.5 McFarland concentration was prepared. All teeth
were mounted in plastic vial caps containing Muller Hinton broth and then exposed to
