Author/Authors :
Arash Ghodousi, A Department of Microbiology - School of Medicine - Tehran University of Medical Sciences, Tehran , Vatani, S Dept. of Microbiology - School of Medicine & Infectious and Tropical Diseases Research Centre - Ahvaz Jundishapur University of Medical Sciences, Ahvaz , Darban-Sarokhalil, Davood Department of Microbiology - School of Medicine - Tehran University of Medical Sciences, Tehran , Omrani, Maryam Department of Microbiology - School of Medicine - Tehran University of Medical Sciences, Tehran , Fooladi, AA Department of Pathology - School of Medical Sciences - Baqiyatallah University of Medical Sciences, Tehran , Khosaravi, AD Dept. of Microbiology - School of Medicine & Infectious and Tropical Diseases Research Centre - Ahvaz Jundishapur University of Medical Sciences, Ahvaz , Feizabadi, Mohammad Mehdi Department of Microbiology - School of Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to clinical isolates of Mycobacterium tuberculosis complex to reduce the cost of using lyticase. This protocol reduces the expense of PFGE typing of Mycobacterium tuberculosis complex as it removes the use of lyticase during the spheroplast formation from these bacteria.
