Author/Authors :
Esmaeilli, Davoud Department of Bacteriology - Faculty of Medical Sciences - Tarbiat Modares University , Mohabati Mobarez, Ashraf Department of Bacteriology - Faculty of Medical Sciences - Tarbiat Modares University , Hatef Salmanian, Ali , Zavaran Hosseini, Ahmad Dept. of Immunology - Faculty of Medical Science - Tarbiat Modares University, Tehran
Abstract :
Background and Objectives: Helicobacter pylori is the causative agent of peptic ulcer disease and a co-factor in development
of gastric malignancies. LPS are among toxic substances produced by H. pylori exhibiting low endotoxic activity compared
to typical bacterial LPS. The aim of this study was to investigate bioactivity of LPS produced by different serotypes of
Helicobacter pylori compared to Escherichia coli and Brucella abortus LPS.
Materials and Methods: Bacterial LPS was extracted by the hot phenol-water method. Biological activities of LPS were
determined via the limulus lysate assay, pyrogenic assay, and blood pressure and PBMC induction test in rabbits.
Results: Biological activity of O2 serotype LPS of H. pylori was less than the biological activity of other H. pylori serotypes.
Conclusion: Our data supported the hypothesis that the unique bacterial LPS of the O2 serotype must be included in the
formulation of a multivalent H. pylori vaccine.
