Author/Authors :
Bagheri, Abouzar Genetic Research Center - University of Social Welfare and Rehabilitation Sciences, Tehran, Iran , Khorram Khorshid, Hamid Reza Genetic Research Center - University of Social Welfare and Rehabilitation Sciences, Tehran, Iran , Mowla, Javad Department of Molecular Genetics - Faculty of Biological Sciences - Tarbiat Modares University, Tehran, Iran , Mohebbi, Hassan Ali Trauma Research Center - Baghiyatallah University of Medical Sciences, Tehran, Iran , Mohammadian, Azam Department of Chemistry - Faculty of Science - Ferdowsi University of Mashhad, Mashhad, Iran , Yaseri, Mehdi Department of Epidemiology and Biostatistics - Tehran University of Medical Sciences, Tehran, Iran , Solaymani-Dodaran, Masoud Iran University of Medical Sciences, Tehran, Iran , Sherafatian, Masih Department of Molecular Genetics - Faculty of Biological Sciences - Tarbiat Modares University, Tehran, Iran , Tavallaie, Mahmood Human Genetic Research Center - Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract :
Background: Diagnosis of Non-small Cell Lung Cancer (NSCLC) at an early stage is a
daunting challenge due to the deficiency of specific noninvasive markers. MicroRNAs
(miRNAs) play important roles in the initiation and progression of NSCLC. Measuring
miRNA expression levels could provide a potential approach for the diagnosis of
NSCLC. Our goals were to examine miR-223, miR-212, miR-192, miR-3074, SNORD33
and SNORD37 expression levels in tissue and sputum of NSCLC patients and cancer
free subjects for molecular diagnosis of NSCLC.
Methods: Relative expressions of miR-223, miR-212, miR-192, miR-3074, SNORD33
and SNORD37 were examined with quantitative real-time RT-PCR assay in tissue
and sputum obtained from 17 NSCLC patients and 17 controls.
Results: miR-3074 was upregulated in tissue samples of NSCLC patients compared
with control group. miR-223 was upregulated, miR-212 and SNORD37 were downergulated
in sputum samples of patients compared with controls. miR-223 quantification
produced 82% sensitivity and 95% specificity with areas under the ROC curve at
0.90 in detection of NSCLC.
Conclusion: miR-223 clearly discriminated cancer patients from cancer-free subjects
and our results suggest that miR-223 could be a diagnostic useful biomarker. The
measurement of altered miRNA expression in sputum samples manifested the potential
noninvasive approach for detection of lung cancer.
