Author/Authors :
Purwoko, Reza Y érpour Medical-Spa Skin and Mesotherapy Centre, Jakarta, Indonesia , Rosliana, Iis érpour Medical-Spa Skin and Mesotherapy Centre, Jakarta, Indonesia , Sobariah, Siti érpour Medical-Spa Skin and Mesotherapy Centre, Jakarta, Indonesia , Hermana, Nabila érpour Medical-Spa Skin and Mesotherapy Centre, Jakarta, Indonesia , Permatasari, Silvani érpour Medical-Spa Skin and Mesotherapy Centre, Jakarta, Indonesia , Wulandari, Dewi Department of Pharmacy - Faculty of Medicine - Universitas Indonesia, West Java, Indonesia , Sari, Puji Department of Biology - Faculty of Medicine - Universitas Indonesia, West Java, Indonesia , Purwaningsih, Ernie H Department of Pharmacy - Faculty of Medicine - Universitas Indonesia, West Java, Indonesia , Chaidir Agro Industrial Technology Development Biomedical Laboratory, Serpong, South Tangerang, Indonesia , Freisleben, Hans-Joachim German Indonesian Medical Association, Jakarta, Indonesia , Pawitan, Jeanne A Department of Histology - Faculty of Medicine - Universitas Indonesia, West Java, Indonesia , Bramono, Kusmarinah Department of Dermatovenerology - Faculty of Medicine - University of Indonesia, West Java, Indonesia
Abstract :
Background: Recently, Phosphatidylcholine (PC) has been used as an off-label treatment
for lipolysis injection, which is associated with inflammatory reaction due to sodium
deoxycholate, an emulsifier, so that inflammation as side effect occurs in those
patients. Liposome formulation from soybean lipid was thought to be a better and
safer alternative. This study aimed to analyze the mechanism of Liposomal Soybean
Phosphatidylcholine (LSPC) extract from Indonesian soybeans (containing 26% PC) to
induce Adipose-derived Stem Cells (ASCs) death in vitro.
Methods: Liposomes were prepared using thin film hydration method followed by a
stepwise extrusion process to produce a small amount of 41.0-71.3 nm. Liposomal soybean
phosphatidylcholine extract (LSPCE), liposomal purified PC (LPCC), and solution
of PC+SD were used for comparison. Annexin V-fluorescein Isothiocyanate/
Propidium Iodide (FITC/PI) double staining by flow cytometry and also measurement
of caspase-3 activity using ELISA were used to quantify the rate of apoptosis. ASCs
viability was measured using MTT assay after induction with liposomes. Morphological
changes were shown using a phase-contrast, inverted microscope and Transmission-
Electron Microscope (TEM).
Results: The flow cytometry results showed that cells treated with both LSPCE and
LPCC showed increase in early apoptosis beginning at 6 hr after incubation, which
was confirmed by caspase 3 measurement. MTT assay showed that both LSPCE and
LPCC could decrease viability of cells. Cells treated with LSPCE and LPCC showed
some rounded cells, which was an early sign of cell death. Cells treated with SD
showed extensive membrane damage with necrosis features using TEM.
Conclusion: The results above demonstrated that LSPCE induced apoptosis of ASCs
