Author/Authors :
Kachuei, Reza Molecular Biology Research Center - Baqiyatallah University of Medical Sciences, Tehran, Iran , Rezaie, Sassan Department of Medical Parasitology and Mycology - Faculty of Public Health - Tehran University of Medial Sciences, Iran , Yadegari, Mohammad Hossein Department of Medical Mycology - Faculty of Medicine Science - Tarbiat Modares University, Tehran, Iran , Safaie, Naser Department of Plant Pathology - Faculty of Agriculture - Tarbiat Modares University,Tehran, Iran , Allameh, Abdol-Amir Department of Biochemistry - Faculty of Medicine Science - Tarbiat Modares University, Tehran, Iran , Aref-poor, Mohammad-Ali Department of Biology - basic science Research - Imam hossein University, Tehran, Iran , Imani Fooladi, Abbas-Ali Applied Microbiology Research Center - Baqiyatallah University of Medical Sciences, Tehran, Iran , Riazipour, Majid Department of Medical Parasitology and Mycology - Faculty of Medicine - Baqiyatallah University of Medial Sciences, Iran
Abstract :
T-2 toxin is the most poisonous trichothecene produced by Fusarium species especially F.sporotrichioides. T-2 toxin is a biological contaminant in a number of agricultural commodities that can cause severe diseases among humans and animals and even lead to death. The aim of the current study is the analysis of T-2 mycotoxin in Fusarium species by high-performance liquid chromatography (HPLC) combined with florescence detection and derivatization with 1-antroylnitrile (1-AN). Totally, 11Fusariumisolates and reference strains were studied. The isolates were tested for the T-2 toxin production after growing on rice substrate followed by using specific “Multisep 225 Trich Clean up columns” purification. In this study, T-2 toxin production was ranged from 197.05ug/kg to 8503.07ug/kg. This is the first study of T-2 toxin analysis by HPLC-F in Iran.
Keywords :
Fusarium , Trichothecene , T-2 toxin , HPLC-F
