Author/Authors :
Ramezani, Reihaneh Department of Biomedical Sciences - Women Research Center - Alzahra University, Tehran, Iran , Kardoost Parizi, Zahra Department of Biotechnology - Faculty of Biological Sciences - Alzahra University, Tehran, Iran , Ghorbanmehr, Nassim Department of Biotechnology - Faculty of Biological Sciences - Alzahra University, Tehran, Iran , Mirshafiee, Hamideh Department of Microbiology - Faculty of Biological Sciences - Shahid Beheshti University, Tehran, Iran
Abstract :
Background: To improve urinary tract infection detection, we evaluated the specificity
and sensitivity of Loop-mediated isothermal Amplification Method (LAMP) for detection
of the Eschericia coli (E. coli) in urine samples, for the first time.
Methods: Primers were designed to target the malB gene of Escherichia coli. LAMP
assay was performed on urine specimens collected from patients with urinary tract infection
symptoms.
Results: As expected, LAMP was more specific and sensitive than direct microscopic
tests. LAMP assay showed the best detection limit of DNA copies with 1.02 copies.
Conclusion: LAMP method offers several advantages in terms of sensitivity, rapidness
and simplicity for detection of E. coli infection in urine samples. The LAMP method
would be highly suitable for the early detection of the UTIs and also comfort quick diagnosis
of UTI in clinical laboratories with limited equipment.
Keywords :
Urinary tract infection , LAMP , Isothermal amplification , Escherichia coli
