Author/Authors :
Bineshian, Farahnaz Department of Parasitology & Mycology - Faculty of Medicine - Semnan University of Medical Sciences, Semnan, Iran , Hosseini, Asieh Blood Transfusion Research Center - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Sharifi, Zohre Blood Transfusion Research Center - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Aghaie, Afsaneh Blood Transfusion Research Center - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
Abstract :
Background: Mutations in the coding region of the Chemokine Receptor 5 (CCR5)
genes reduce or eliminate CCR5 expression in immune cells and progression of HCV infection.
This study aimed to investigate the role of this mutation in HCV infection in
Iranian patients in comparison with healthy individuals.
Methods: 100 HCV infected patients and 100 healthy individuals were randomly selected.
The CCR5Δ32 genotypes were determined using specific primers and PCR method.
Results: The agarose gel electrophoresis showed a189-bp fragment from wild type for
both alleles of CCR5 gene. The CCR5-Δ32 allele was not found in any HCV infected
and healthy subjects.
Conclusion: The mutation in CCR5 gene was not detected in any of the two groups;
therefore, the role of CCR5 gene expression in immune cells and progression of HCV
infection needs to be studied in larger samples in our country.
Keywords :
Mutation , Infection , Human , Hepatitic C , CCR5 protein
