Author/Authors :
Ghorbian, Saeid Department of Molecular Genetics - Ahar Branch Islamic Azad University, Ahar, Iran , Nargesian, Mansooreh Young Researchers and Elite Club - Ahar Branch Islamic Azad University, Ahar, Iran , Talaneh, Sasan Department of Molecular Genetics - Ahar Branch Islamic Azad University, Ahar, Iran , Asnaashari, Omid Department of Radiation Oncology - Omid Hospital - Western Azerbaijan University of Medical Sciences, Urmia, Iran , Sharifi, Rasol Department of Biology - Ahar Branch Islamic Azad University, Ahar, Iran
Abstract :
Background: Recently, findings have validated the significant role of DNA damage genes related to the pathogenesis of breast
cancer (BC). The aim of the present investigation was to evaluate possibility roles of two common XRCC1 (rs25487; A > G) and ERCC1
(rs3212964; A > G) gene polymorphisms with the risk of sporadic BC.
Methods: In a case-control study, consisting of 100 females identified with sporadic BC and 100 malignancy-free females as the
control group. We used Tetra-ARMS Polymerase Chain Reaction (PCR) and PCR-Restriction Fragment Length Polymorphism (RFLP)
methods to determine genotype frequencies of XRCC1 and ERCC1 genes.
Results: The findings did not reveal a statistically significant difference in the genotype frequencies of XRCC1 and ERCC1 genes between
the two groups (P > 0.05). The frequency of G mutant allele for XRCC1 and ERCC genes was higher in cases compared to
controls, while the difference between the groups was not statistically significant (P = 0.202; OR: 1.312; CI: 0864 - 1.994), (P = 0.352; OR:
1.213; CI: 0.808 - 1.820).
Conclusions: The current results provide evidence against the hypothesis that XRCC1 (rs25487) and ERCC1 (rs3212964) gene polymorphisms
may be associated with a predisposition to sporadic BC.
Keywords :
DNA Repair , ERCC1 , Breast Cancer , XRCC1