Design and Fabrication of PVC Membrane Electrode Based on Netural Ligand (E)- N'-1-2-Hydroxyphenyl Ethylidene) Benzohydrazi for Teramadol hydrochloride Measurement in Drugs and Biological Fluids

Here, a simple, quick and sensitive potentiometric method is described for tramadol hydrochloride measurement using PVC membrane electrode based on neutral ligand (E)-N'-(1-(2-hydroxyphenyl)ethylidene)benzohydrazid. Besides, effect of various parameters like ionic additives, membrane solvent and pH on the potentiometric response of the electrode are investigated. The best electrode performance was observed with membrane composition of PVC 30%, DBP 60%, carrier 7% and additive 3% within pH range of 3-6. This electrode showed Nernstian slope about -60.24 ± 0.2 mV dec-1, detection limit of 1.75 × 10-6 M and response time about 15 s over the concentration range of 1.0 × 10-1-3.16 × 10-6 M. The lifetime of electrode was about 40 days. Finally, to evaluate the performance of the electrode, the amount of tramadol hydrochloride were measured in three samples, including urine, cow's milk and tablet. The results showed that the mentioned electrode has the more appropriate performance compared to the other usual approaches.