Title of article :
IL-10 and IL-12 Production in Response to Mycobacterium Tuberculosis Total Lipid Antigens in Multidrug-Resistant Tuberculosis
Author/Authors :
Shahemabadi, Ali Shams Department of Immunology - Faculty of Medical Sciences - Shaheed Sadughi University of Medical Sci-ences - Yazd , Zavaran Hossein, Ahmad Department of Immunology - Faculty of Medical Sciences - Tarbiat Modares University - Tehran , Shaghasempour, Shapour National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Masih Daneshvari Hospital - Beheshti University of Medical Sciences - Tehran , Masjedi, Mohammad Reza National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Masih Daneshvari Hospital - Beheshti University of Medical Sciences - Tehran , Rayani, Majid National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Masih Daneshvari Hospital - Beheshti University of Medical Sciences - Tehran , Shams, Majid National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Masih Daneshvari Hospital - Beheshti University of Medical Sciences - Tehran , Esphandyari, Nasrin National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Masih Daneshvari Hospital - Beheshti University of Medical Sciences - Tehran , Pouramiri, Majid National Research Institute of Tuberculosis and Lung Diseases (NRITLD) - Masih Daneshvari Hospital - Beheshti University of Medical Sciences - Tehran
Abstract :
Background: Mycobacterium tuberculosis lipid antigens take part in pathogenicity of the bacterium but the response of monocytes/macrophages to these antigens in tubercu-losis is not well known. Objective: The aim of current investigation was to study the M. tuberculosis lipid antigens in tuberculosis pathogenesis. Methods: In the present study M. tuberculosis lipid antigens were extracted. Monocytes and macrophages from mul-tidrug-resistant tuberculosis (MDR-TB), TB patients, asymptomatic healthy individuals with positive tuberculin skin test positive and healthy individuals with negative tubercu-lin skin test were collected using magnetic cell sorting. The cells were stimulated by M. tuberculosis total lipid antigens and IL-12 and IL-10 in their supernatants were meas-ured by enzyme-linked immunosorbent assay. Results: The IL-12 production by mono-cytes in response to M. tuberculosis total sonicate antigens in the MDR-TB patients did not show a considerable difference with the PPD positive healthy subjects, whereas in the active TB patients, IL-12 levels significantly decreased (p<0.05). IL-10 production by monocytes in TB patients in response to total lipid antigens showed a significant in-crease in comparison to MDR-TB patients and healthy individuals. Conclusion: In the MDR-TB patients, IL-10 and IL-12 production by monocytes in response to M. tubercu-losis lipid antigens are similar to the healthy subjects.
Keywords :
Multidrug Resistant Tuber-culosis , Monocyte , Macrophage , Lipid , IL-12 , IL-10
Journal title :
Astroparticle Physics
