Title of article
Rapamycin Inhibits Expansion of Cord Blood Derived NK and T Cell
Author/Authors
Zare, Monireh Tabriz University of Medical Sciences - Tabriz , Valipour, Behnaz Tabriz University of Medical Sciences - Tabriz , Mohammadi, Seyede Momeneh Tabriz University of Medical Sciences - Tabriz , Nouri, Mohammad Tabriz University of Medical Sciences - Tabriz , Movassaghpour, Aliakbar Tabriz University of Medical Sciences - Tabriz , Charoudeh, Hojjatollah Nozad Tabriz University of Medical Sciences - Tabriz
Pages
8
From page
192
To page
199
Abstract
Background: The mammalian target of rapamycin (mTOR) is important in
hematopoiesis. Despite the central role of mTOR in regulating the differentiation of
immune cells, the effect of mTOR function on cord blood mononuclear cells is yet to be
defined. Objectives: To evaluate the effect of mTOR inhibition, using rapamycin on the
proliferation and apoptosis of cord blood mononuclear cells, as well as on the B and T
cell expansion. Methods: Cord blood mononuclear cells were cultured in the presence
of IL-2, IL-7 and IL-15 cytokines and inhibited by rapamycin for 14 days. The
harvested cells were evaluated at distinct time points by flow cytometry. Results: The
mTOR expression decreased in the presence of rapamycin on day 14. Inhibition of
mTOR reduced the proliferation of the cord blood mononuclear cells, yet did not
influence apoptosis. Moreover, the number of T and NK cells was significantly reduced
in the presence of rapamycin, while no change was observed in the B cell expansion.
Conclusion: mTOR signaling plays a crucial part in cord blood derived NK and T cells
expansion.
Keywords
Cytokine , Rapamycin , NK and T Cells , mTOR
Journal title
Astroparticle Physics
Serial Year
2017
Record number
2429413
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