Title of article
Evaluation of Genetic Pattern of Gentamicin-resistant Enterococci Isolated from Clinical Samples
Author/Authors
Balaei Gajan, Esrafil Department of Community Dentistry - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz , Akbarzadeh, Parisa Department of Community Dentistry - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz , Harasi, Bahram Department of Oral and Maxillofacial Pathology - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz , Moosavi, Zohreh Microbiology Laboratory - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz
Pages
4
From page
23
To page
26
Abstract
Background and aims. Enterococci are among resistant microorganisms to antibiotics and are responsible for some of acute gingival infections. This study aimed at molecular evaluation of gentamicin-resistant enterococcus species and deter-mining the resistance genes.
Materials and methods. Clinical samples were cultured on BHI medium and enterococci were isolated by specific methods and antibiograms were prepared. DNA was derived from gentamicin-resistant species by alkaline lysis method and replicated by PCR method. Four gentamicin-resistant genes were evaluated by electrophoresis method on agarose gel.
Results. From 105 Enterococcus faecalis isolates, 36.2% and from 38 Enterococcus faecium isolates, 78.2% were resistant to gentamicin. 60% of these species bore aac (6’)- le – aph(2”)- la gene. ph(2”)-Ib,Ic,Id genes were not seen in any of the evaluated species.
Conclusion. In gentamicin-resistant species of E. faecalis and E. faecium, the aac (6’)- le – aph(2”)- la gene was identi-fied as the main cause of resistance.
Keywords
gentamicin , enterococcus , Antibiogram
Journal title
Astroparticle Physics
Serial Year
2011
Record number
2429830
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