Author/Authors :
Balaei Gajan, Esrafil Department of Community Dentistry - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz , Akbarzadeh, Parisa Department of Community Dentistry - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz , Harasi, Bahram Department of Oral and Maxillofacial Pathology - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz , Moosavi, Zohreh Microbiology Laboratory - Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz
Abstract :
Background and aims. Enterococci are among resistant microorganisms to antibiotics and are responsible for some of acute gingival infections. This study aimed at molecular evaluation of gentamicin-resistant enterococcus species and deter-mining the resistance genes.
Materials and methods. Clinical samples were cultured on BHI medium and enterococci were isolated by specific methods and antibiograms were prepared. DNA was derived from gentamicin-resistant species by alkaline lysis method and replicated by PCR method. Four gentamicin-resistant genes were evaluated by electrophoresis method on agarose gel.
Results. From 105 Enterococcus faecalis isolates, 36.2% and from 38 Enterococcus faecium isolates, 78.2% were resistant to gentamicin. 60% of these species bore aac (6’)- le – aph(2”)- la gene. ph(2”)-Ib,Ic,Id genes were not seen in any of the evaluated species.
Conclusion. In gentamicin-resistant species of E. faecalis and E. faecium, the aac (6’)- le – aph(2”)- la gene was identi-fied as the main cause of resistance.
