Author/Authors :
Niaei, Gholamreza Department of Biology - Faculty of Sciences - University of Guilan, Rasht , Heidaryan, Somayeh Department of Biology - Faculty of Guilan Pardis - Department of Biology - University of Guilan, Rasht , Niaei, Alireza Department of Medicine - School of Medicine - Urmia University of Medical Sciences, Urmia , Didevar, Nasim Alsadat Department of biology - East Azerbaijan Science and Research Branch - Islamic Azad University, Tabriz
Abstract :
Endometriosis is considered as a multifactorial disease that affects up to 10% of all women of fertile age.
Vascular endothelial growth factor (VEGF) is one of the most important activators of angiogenesis. VEGF
is known to be a key molecule in the pathogenesis of endometriosis. A great number of studies have
referred to genetic polymorphisms as a factor that contributes to the development of endometriosis. The
present study was aimed to find out the frequency of the VEGF -1154G/A polymorphism and its
relationship with endometriosis risk in Iranian women with endometriosis.This study involved 175 patients
with endometriosis and 131 healthy controls. Following extraction of genomic DNA from patients and
controls, genotyping of the -1154G/A polymorphism of the VEGF gene were performed by polymerase
chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Multivariate analysis
was used to examine the association between the risk of endometriosis and VEGF -1154G/A
polymorphism. There was statistically a significant difference in the frequency of the -1154G/A
polymorphism between patients and the controls. The percentage distribution of the three -1154G/A
genotypes was (GG 3.4%, GA %46.8%, AA 49.7%) in endometriosis patients.
Conclusion: Data supports the hypothesis that angiogenesis is of pivotal importance in the development of
endometriosis. Our study indicates that there is significant association between the VEGF gene -1154G/A
polymorphism and the risk of endometriosis in North West of Iran.
