Author/Authors :
Arababadi, Mohammad Kazemi Molecular-Medicine Research Center - Rafsanjan University of Medical Sciences, Rafsanjan , Hassanshahi, Gholamhossein Molecular-Medicine Research Center - Rafsanjan University of Medical Sciences, Rafsanjan , Rezazadeh Zrandi, Ebrahim Molecular-Medicine Research Center - Rafsanjan University of Medical Sciences, Rafsanjan , Pourfathollah, Ali Akbar Department - of Immunology - School of Medicine - Tarbiat Modares University, Tehran , Daneshmandi, Saeed Department - of Immunology - School of Medicine - Tarbiat Modares University, Tehran , Shamsizadeh, Ali Department of Physiology - School of Medicine - Rafsanjan University of Medical Sciences, Rafsanjan , Asiabanha Rezaei, Majid Department of Biochemistry - School of Medicine - Rafsanjan University of Medical Sciences, Rafsanjan , Eigder, Somayeh Department of Biochemistry - School of Medicine - Rafsanjan University of Medical Sciences, Rafsanjan
Abstract :
Objective(s)
Although, type 2 diabetes is the most frequent type of diabetes, its main cause is yet to be clarified. Several
environmental and genetic parameters are believed to be involved in diabetes. It has also been established
that cytokines play key roles in pathogenesis of diabetes. Expression of cytokines is different from person to
person and in different societies. Several studies showed that polymorphisms of +874 of interferon-gamma
(IFN-γ) and -590 of interleukin-4 (IL-4) are associated with the regulation of expression of these genes. This
study was aimed to find polymorphisms of these regions in type 2 diabetes patients.
Materials and Methods
In this experimental study peripheral blood samples were collected from 160 type 2 diabetic patients and 160
healthy controls. DNA was extracted by salting out method. Polymorphisms of +874 of IFN-γ and -590 of
IL-4 were analyzed by ARMS-PCR and RFLP-PCR.
Results
Our findings indicated that TT genotype of IFN-γ was increased in type 2 diabetic patients compared to the
control but difference was not significant. Our results didn’t show any significant difference between IL-4
genotype in diabetic and healthy controls either.
Conclusion
Our results suggested that TT genotype of IFN-γ can be associated with diabetes. This association can be
described by the fact that over expression of IFN-γ shifts immune system to Th1; therefore, pancreatic cells
can be miscarried by immune cells.
Keywords :
Type 2 diabetes , Polymorphism , Interleukin-4 , Interferon-gamma
