Can atrial natriuretic peptides measurement diagnose heart failure at different age groups?

With interest, I read the article by Khalilian et al.1 entitled "Relationship between echocardiographic findings and laboratory serum biomarkers in patients with and without low cardiac output" that recently published in your valuable journal. We know that in adult patients, atrial natriuretic peptides (ANP) and brainnatriuretic peptides (BNP) have been used for diagnosis, monitoring of treatment effects, and estimating the prognosis of systolic and diastolic heart failure. 2 Furthermore, ANP would remain quite stable during storage in plasma at −80 °C for even 12 months.3 I would like to attract the authors' and readers' attention to some points that can be helpful in conducting future studies.