Title of article :
Antithrombotic effect of ligustrazine hydrochloride injection on the model of induced arteriovenous shunt thrombosis
Author/Authors :
Wang, Peng Department of Clinical Laboratory - The First College of Clinical Medical Science - China Three Gorges University, Yichang , Luo, Chun-hua Department of Clinical Laboratory - The First College of Clinical Medical Science - China Three Gorges University, Yichang , Wang, Qun-xing Department of Clinical Laboratory - The First College of Clinical Medical Science - China Three Gorges University, Yichang , Li, Qian-yuan Department of Clinical Laboratory - The First College of Clinical Medical Science - China Three Gorges University, Yichang , Li, Peng Department of Clinical Laboratory - The First College of Clinical Medical Science - China Three Gorges University, Yichang , Yuan, Xiao-hui Institute of Biomedicine - Jinan University - Guangzhou, China
Abstract :
Background: The objective of this study is to optimize the effective dose of heparin and ligustrazine hydrochloride injection (LHI)
for drug combination. Materials and Methods: The animal clinical study of LHI was performed by the rat’s model of induced
arteriovenous shunt thrombosis. Experimental animals were grouped into several groups and separately treated with both LHI
(20, 40, 80 mg/kg, i.p.) and heparin (60, 55, and 50 U/kg; 5000 U/ml; Sigma, i.v). The study had used thrombus weight, protein
concentration in thrombus homogenate, inhibition rate of thrombosis, and plasma anti-thrombin activity as indications. Results:
The group combination (50, 80) got the result of 100% antithrombotic activity with 0 ± 0 mg of thrombus weight, 14 ± 3 μg/ml of
protein concentration in thrombus homogenate and 1.5 ± 0.04 U/ml of plasma anti-thrombin activity. Its anti-thrombotic effect
was much better than individual groups treated with LHI in a dose of 0 mg/kg and group of combination (0, 80) (P < 0.05) while
antithrombotic effect of 55 and 60 U/kg heparin alone was only 37-58%. Therefore, the group of combination (50, 80) was optimal
for 100% antithrombotic activity. Conclusion: Optimal combined doses of LHI and heparin preventing blood coagulation were
determined and the results were available. It may give some hint for the further clinical application on human.
Keywords :
Anti-thrombotic , heparin , ligustrazine hydrochloride injection
Journal title :
Astroparticle Physics
