Effect of Different Concentrations and Application Methods of Polyamines (Putrescine, Spermine, Spermidine) on Some Morphological, Physiological, and Enzymatic Characteristics and Vase Life of Rosa hybrida cv. ‘Dolce Vita’ Cut Flower

In order to study the effect of different application methods of polyamines (putrescine, spermine, spermidine) on some morphological, physiological and enzymatic traits and vase life of Rosa hybrida cv. ‘Dolce Vita’, as a factorial experiment was conducted in a completely randomized design with 32 treatments, 3 replicates and each replication containing 5 vases, amounting to a total of 480 vases. The first factor was assigned to putrescine, spermine, spermidine (50 or 100 mg/l) and the second factor was devoted to four application methods (pre-harvest spray, postharvest spray, 24 hrs pulse, and continuous). We applied two blanks in the experiment: distilled water and 3% sucrose + 3 mg/l nanosilver. Morphological, physiological and enzymatic traits, such as relative fresh weight, cell membrane stability index, anthocyanin of petals, total chlorophyll of leaves, protein, phenylalanine ammonia- lyase, superoxide dismutase enzyme activity, and vase life, were measured. The results showed that 100 mg/l Spm in pre-harvest spray method was related to the highest rates of protein, activity of phenylalanine ammonia-lyase, and superoxide dismutase enzymes. Therefore, pre-harvest spraying of polyamines can be recommended as the best way to improve morphological, physiological and enzymatic traits as well as the vase life of Rosa hybrida cv. ‘Dolce Vita’.