Author/Authors :
Vakili, Bahareh Department of Microbiology - Science and Research Branch - Islami Azad University, Kerman , Khorvash, Farzin Isfahan University of Medical Sciences, Isfahan , Fazeli, Hossein Department of Microbiology and Infectious Diseases and Tropical Medicine Research Center - Isfahan University of Medical Sciences, Isfahan , Khaleghi, Moj Department of Biology - Faculty of Science - Shahid Bahonar University of Kerman, Kerman
Abstract :
Background: Th e purpose of this study was to screen of parC gene mutations in clinical isolates of Acinetobacter baumannii from
intensive care units (ICUs) of Alzahra Hospital, Isfahan, Iran. Materials and Methods: Seventy isolates of A. baumannii between
March 2011 and June 2012 were studied. Susceptibility test was established by E-test method. Polymerase chain reaction-restriction
fragment length polymorphism (PCR-RFLP) and sequencing was performed for detection of parC gene mutation. Results: 77.1% of
specimens were highly resistant. Mutation at position 80 in parC was observed in 93% of isolates. Conclusion: High proportion of
A. baumannii isolates had a mutation in parC that can play an important role in increased incidence of these isolates.
