Author/Authors :
Lasom, Supakanya Graduate School - Khon Kaen University, Thailand , Komanasin, Nantarat Khon Kaen University, Thailand , Settasatian, Nongnuch Khon Kaen University, Thailand , Settasatian, Chatri Khon Kaen University, Thailand , Kukongviriyapan, Upa Khon Kaen University, Thailand
Abstract :
Background: The imbalance of von Willebrand factor (vWF) and a disintegrin and metalloproteinase with a thrombospondin type 1
motif member 13 (ADAMTS13) has been associated with atherosclerosis progression. A high level of vWF which regulates thrombus
formation is associated with diabetes mellitus (DM), and some ADAMTS13 and vWF polymorphisms have effects on their levels.
Therefore, this study aimed to evaluate the associations of ADAMTS13 and vWF polymorphisms and their levels with DM and severity
of coronary stenosis. Materials and Methods: Eighty‑seven DM and 84 control individuals were recruited. vWF and ADAMTS13
activities as well as vWF antigen were measured by collagen‑binding assay (CBA), residual‑CBA, and in‑house enzyme‑linked
immunosorbent assay, respectively. ADAMTS13 and vWF polymorphisms were determined by polymerase chain reaction‑restriction
fragment length polymorphism. Results: The E and G alleles and AA genotype of ADAMTS13 Q448E, rs2073932, and rs652600,
respectively, were independently associated with DM (odds ratio [OR] [95% confidence interval (CI)] = 2.5 [1.1, 5.6], 2.3 [1.0, 5.2],
and 4.7 [1.2, 18.6], respectively). Moreover, E allele and AA genotype of Q448E and rs652600 were also significantly associated with
multi‑vessel disease (OR [95% CI] = 2.2 [1.0, 4.8] and 3.2 [1.0, 10.0], respectively), while the E and G allele of Q448E and rs2073932
were associated with high Gensini score (OR [95% CI] = 2.3 [1.1, 4.9] and 2.3 [1.1, 5.1], respectively). Conclusion: Association of
ADAMTS13 polymorphisms with DM, number of vessel stenosis, and Gensini score may indicate the possible contribution of
ADAMTS13 polymorphisms to atherosclerosis progression and severity of coronary stenosis in DM.
Keywords :
A disintegrin and metalloproteinase with a thrombospondin type 1 motif member 13 , coronary stenosis , diabetes mellitus , polymorphism