Author/Authors :
Nicknam, Mohammad Hossein Immunology Department - Faculty of Medicine - Tehran University of Medical Sciences, Tehran , Torkashvand, Ali Immunology Department - Faculty of Medicine - Tehran University of Medical Sciences, Tehran , Ghods, Ahmad Transplantation Department - Hashemi nejad Hospital - Iran University of Medical Sciences, Tehran , Amirzargar, Ali Akbar Immunology Department - Faculty of Medicine - Tehran University of Medical Sciences, Tehran , Amirkhani, Aref Epidemiology Department - Institute Pasteur, Tehran , Khosravi, Farideh Immunology Department - Faculty of Medicine - Tehran University of Medical Sciences, Tehran , Nikbin, Behrouz Immunology Department - Faculty of Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
Studies have shown that patients who do not produce donor specific and / or panel
reactive anti-HLA antibodies have a longer graft survival. The purpose of this study
was to evaluate the posttransplant humoral immune response towards HLA-class I
antigens and the measurement of the serum creatinine levels which are used in
monitoring posttransplant function of kidney.
Serum samples from 132 renal transplant recipients were screened for preformed
anti-HLA class I panel reactive antibodies (PRA) by means of microlymphocytotoxicity
assay.
The results revealed the presence of PRA in 26 (19.7%) out of 132 transplanted
patients. Graft function was evaluated by measurement of serum creatinine levels
which revealed the mean of 1.75 mg/dl (SD: 1.08). Because of clinical significance of
presence of different PRA amounts (>10%, > 20% and >50% of panel reactivity) in
patients, correlation with kidney function status was analyzed. The obtained data
highlighted a higher presence of serum creatinine levels in PRA-positive patients
compared to negative patients (P<0.01).
These results (and further studies for class II, …) can be used to implement new
therapeutic strategies to curtail post transplant alloantibodies production and better
allografts survival
