Author/Authors :
Ravanbakhsh, Masoud Department of Immunology - School of Public Health and Institute of Public Health Research - Medical Sciences/University of Tehran, Tehran , Sarafnejad, Abdolfatah Department of Immunology - School of Public Health and Institute of Public Health Research - Medical Sciences/University of Tehran, Tehran , Aghamohammadi, Asghar Department of Allergy and Clinical Immunology - Children’s Medical Center - Medical Sciences/University of Tehran, Tehran , Kardar, Gholam Ali Department of Allergy and Clinical Immunology - Children’s Medical Center - Medical Sciences/University of Tehran, Tehran , Asgarian Omran, Hossein Department of Immunology - School of Public Health and Institute of Public Health Research - Medical Sciences/University of Tehran, Tehran , Atarod, Lida Medical Sciences/University of Tehran, Tehran , Rezaei, Nima Department of Allergy and Clinical Immunology - Children’s Medical Center - Medical Sciences/University of Tehran, Tehran , Shahrestani, Tahereh Department of Immunology - School of Public Health and Institute of Public Health Research - Medical Sciences/University of Tehran, Tehran , Hosseini, Mostafa Department of Epidemiology and Biostatistics - School of Public Health and Institute of Public Health Research - Medical Sciences/University of Tehran, Tehran , Moin, Mostafa Department of Allergy and Clinical Immunology - Children’s Medical Center - Medical Sciences/University of Tehran, Tehran
Abstract :
Common variable immunodeficiency (CVID) is the most common symptomatic primary
antibody deficiency, characterized by reduced serum immunoglobulins levels and increased
susceptibility to recurrent pyogenic infections.
In this study, we evaluated CD40 ligand expression on stimulated versus unstimulated Thelper
lymphocytes of nine Common variable immunodeficient patients in comparison with
fifteen normal controls. Phorbol myristate acetate (PMA) and Ionomycin were used to
stimulate cells in vitro. After six hours stimulation, the cells were subjected to surface staining
with three-color staining procedure. Events were analyzed by flow cytometer, using FloMax
software.
Results were reported as the percentage of lymphocytes expressing CD markers. We did
not find any significant statistical difference in CD40 ligand expression between patients and
controls (p>0.05), despite having stimulation documented by CD69 expression as activation
marker in each run.
The results of this study are in agreement with some other studies, indicating that CD40
ligand expression on stimulated T-helper lymphocytes of Common variable
immunodeficiency patients is similar to normal controls.
