Author/Authors :
Trajkov, Dejan University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Mirkovska-Stojkovikj, Jagoda University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Petlichkovski, Aleksandar University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Strezova, Ana University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Efinska-Mladenovska, Olivija University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Sandevska, Emilija University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Sibinovska, Olgica University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Hristomanova, Slavica University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Djulejic, Eli University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Petrov, Jordan University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia , Gogusev, Jean Hôpital Necker-Enfants Malades - Paris, France , Spiroski, Mirko University School of Medicine "Ss. Kiril and Metodij" - Skopje, Republic of Macedonia
Abstract :
The aim of this study was to examine the association of 22 cytokine gene polymorphism
in Macedonians with chronic obstructive pulmonary disease (COPD).
The sample of the population comprised of 301 normal respondents and 62 patients with
COPD. Cytokine genotyping was performed by polymerase chain reaction with sequencespecific
priming (PCR-SSP).
Positive (susceptible) association was found between patient with COPD and IL-1α -
889/C allele; where as negative (protective) association among was found for the following
alleles IL-1β +3962/C; IL-12B -1188/A; IFNγ +874/T; IL-2 -330/G; IL-4 -1098/G and IL-
4-33/C. We found positive (susceptible) association between patients with COPD and following
genotypes: IL4 -33/T:T; IFNγ +874/A:A; IL-4 -1098/T:T ; IL-1α -889/C:C; IL-1β
+3962/C:T; IL-12B -1188/C:C; IL-4Rα +1902/G:G; IL-10 -1082/G:G; IL-2 -330/T:T; IL-4
-590/C:C; and IL-1α -889/C:T. Negative (protective) association between patients with
COPD and following genotypes was found: IFNγ +874/A:T; IL-4 -33/C:T; IL-4 -1098/G:T;
IL-2 -330/G:T; IL-1β +3962/C:T; IL-4 -590/C:T; IL-10 -1082/A:G; and IL-4 -33/C:C. Positive
(susceptible) association between patients with COPD and following haplotypes was
found: IL-4/TCT; IL-10/ATC; and IL-2/TG, and negative (protective) association was
found between the patients with COPD and haplotypes for: IL-4/TTC; and IL-4/GCC.
It could be concluded that several cytokine polymorphisms are positively (susceptible), or
negatively (protective) associated with COPD in Macedonians.
