Author/Authors :
Mortazavi, Hossein Department of Dermatology - Autoimmune Bullous Diseases Research Center - School of Medicine - Tehran University of Medical Sciences, Tehran , Shahdi, Majid Department of Dermatology - Autoimmune Bullous Diseases Research Center - School of Medicine - Tehran University of Medical Sciences, Tehran , Amirzargar, Ali Akbar Department of Immunology - School of Medicine - Tehran University of Medical Sciences, Tehran , S.Naraghi, Zahra Department of Pathology - School of Medicine - Tehran University of Medical Sciences, Tehran , Valikhani, Mahin Department of Dermatology - Autoimmune Bullous Diseases Research Center - School of Medicine - Tehran University of Medical Sciences, Tehran , Daneshpazhooh, Maryam Department of Dermatology - Autoimmune Bullous Diseases Research Center - School of Medicine - Tehran University of Medical Sciences, Tehran , Vasheghani-Farahani, Amir Department of Dermatology - Autoimmune Bullous Diseases Research Center - School of Medicine - Tehran University of Medical Sciences, Tehran , Sedaghat, Mojtaba Department of Community Medicine - School of Medicine - Tehran University of Medical Sciences, Tehran , Chams-Davatchi, Cheyda Department of Dermatology - Autoimmune Bullous Diseases Research Center - School of Medicine - Tehran University of Medical Sciences, Tehran
Abstract :
Anti-desmoglein 3 and 1 autoantibodies are involved in the pathogenesis of pemphigus
diseases. Our objective was to assess the value of ELISA in the diagnosis of pemphigus and
its correlation with the severity of pemphigus vulgaris.
Based on clinical presentation and histopathologic confirmation for the diagnosis of the
pemphigus, 38 patients took part in the study. Sera of the patients were tested by desmoglein
1 and desmoglein 3 ELISA. Also, direct immunofluorescence was performed for all patients
which revealed positive results in 36 patients (94.7%).
ELISA was positive in 37 of 38 pemphigus patients (Sensitivity: 97.3%). The relationship between
desmoglein 1 index values and skin severity was statistically significant (p<0.05). Desmoglein
3 index values increased with oral severity although this was not statistically significant. Iranian
patients similar to Indian patients had higher positive anti-desmoglein 1 autoantibodies.
Desmoglein-ELISA test is appropriate in the diagnosis of pemphigus. Desmoglein 1 index
value is statistically correlated with the severity of pemphigus vulgaris.
Keywords :
ELISA , Desmoglein 1 , Desmoglein 3 , Pemphigus vulgaris
