Author/Authors :
Ahangar Oskouee, Mahin Department of Microbiology &Virology - Faculty of Medicine - Tabriz University of Medical Sciences, Tabriz , Shahmahmoudi, Shohreh Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , Nategh, Rakhshandeh Department of Virology - School of Public Health - Tehran University of Medical Sciences, Tehran , Ismaili, Heidar-Ali Department of Pathobiology - Tabriz University of Medical Sciences, Tabriz , Safaeyan, Firozeh Department of Microbiology &Virology - Faculty of Medicine - Tabriz University of Medical Sciences, Tabriz , Zareinghalame Moghaddam, Maryam Department of Microbiology - International Branch of Shahid Beheshti Medical University, Tehran , Agazadeh, Mohammad Department of Microbiology &Virology - Faculty of Medicine - Tabriz University of Medical Sciences, Tabriz , Savadi Oskouee, Ayda Faculty of Dentistry - Tabriz University of Medical Sciences, Tabriz
Abstract :
Background: The TP53 gene is the most important tumor suppressor gene in
humans. The aim of our study was to determine the genotype frequency of three
common TP53 polymorphisms (codon 72 BstUI and intron 6 MspI, as well as the
intron 3) in a group of Iranian women with and without breast cancer.
Methods: Paraffin-embedded specimens of 65 malignant breast cancer cases
and 65 cases with benign breast lesions were investigated for the presence of three
common TP53 polymorphisms by polymerase chain reaction. Samples were
genotyped by polymerase chain reaction followed by variant specific restriction
enzyme digestion.
Results: In our study, age grouping as >50 and ≤50 showed that the highest
number of cancerous and non-cancerous patients was in the age group under 50;
according to statistical tests, the difference was significant and recessive alleles of
all three hot spots of TP53 had the highest frequency in the cancerous group. The
majority of the cases with recessive alleles of all three hot spots of TP53 were in
the age group ≤ 50. The difference between cancerous and noncancerous groups
was statistically significant.
Conclusions: Our results indicate that recessive alleles in three hot spots of
TP53 gene might play a role in the breast cancer development, especially in women
younger than 50 years.
